Bioassay-guided isolation of α-glucosidase inhibitors from Byrsonima garcibarrigae Cuatrec.

Introduction:Byrsonima garcibarrigae is an endemic tree of Amazonas state, Brazil, with pharmacological and chemical knowledge poorly understood. Aim: To investigate the antidiabetic potential B. stem bark. Methods: The bark was sequentially extracted by maceration hexane (EHBG), ethyl acetate (EABG), methanol (EMBG). antioxidant capacity, α-glucosidase inhibitory potentials anti-glycation capacities were evaluated. A bio-guided fractionation gave compounds that characterized MS NMR. Results: 8 identified HPLC-MS. EMBG showed highest activity (1.09±0.32 μg/mL), (9.2±0.23 μg/mL) phenolic content (61.43±0.50%), thus fractionated producing (FHX), chloroform (FCL) hydromethanolic (FHM) fractions. After additional anti-α-glucosidase assays, FHM (1.02±0.49 giving quercitrin epicatechin. assay EMBG, presented higher activities in comparison to positive control, aminoguanidine. Conclusions:B. displayed since inhibited α-glucosidase, as well expressive recorded.